Protein-Lipids/Nucleic Acid Interaction Assay and Screening

ChIP (Chromatin Immunoprecipitation) is a type of immunoprecipitation experimental technique used to investigate the interaction between proteins and DNA in the cell. It aims to determine whether specific proteins are associated with specific genomic regions, such as transcription factors on promoters or other DNA binding sites, and possibly defining cistromes. ChIP also aims to determine the specific location in the genome that various histone modifications are associated with, indicating the target of the histone modifiers.

CLIP (cross-linking immunoprecipitation) is a method used in molecular biology that combines UV cross-linking with immunoprecipitation in order to analyze protein interactions with RNA. CLIP-based techniques can be used to map RNA binding sites for a protein of interest on a genome-wide scale, thereby increasing the understanding of post-transcriptional regulatory networks.

EMSA (electrophoretic mobility shift assay) is a common affinity electrophoresis technique used to study protein-DNA or protein-RNA interactions. This procedure can determine if a protein or mixture of proteins is capable of binding to a given DNA or RNA sequence, and can sometimes indicate if more than one protein molecule is involved in the binding complex.

Fat Western is used to screen for protein-lipid interactions. Using a dot-blot of a selected set of lipids one can determine the lipid-binding specificity of a particular target protein. In addition, it can be used to identify the lipid-binding domains within a target protein. Moreover, the assay permits an estimation of the affinity constant. Technically, fat westerns are based on immunoblotting techniques in which lipid-protein binding precedes the antibody detection steps.

Creative BioMart also provides assistance in experimental design, training to conduct assays and data interpretation in protein-lipids/nucleic acid interaction assay and screening. For details, please contact us.

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