Cross-Linking and Immunoprecipitation (CLIP)

Creative Diagnostics can serve full line of CLIP-Seq service. We combine UV cross-linking and immunoprecipitation with high-throughput sequencing to identify binding sites of RNA-binding proteins. CLIP-seq depends on cross-linking induced mutation sites (CIMS) to localized protein-RNA binding sites. Because CIMS are reproducible, high sequencing depths allow CIMS to be differentiated from technical errors.

Besides CLIP-Seq, we also offer following services to meet your research demands.

PAR-CLIP: Photoactivatable-ribonucleoside-enhanced crosslinking and immunoprecipitation attempts to solve some of the problems of CLIP, namely, efficiency of crosslinking and resolution of RBP binding sites.

iCLIP: Individual-nucleotide resolution CLIP is a refinement of CLIP that allows single-nucleotide resolution of RBP binding sites.

miCLIP: Methylation individual-nucleotide-resolution crosslinking and immunoprecipitation is a specialized version of iCLIP designed to determine which RNA nucleotides are methylated by the RNA methyltransferase Nsun2.

Creative Diagnostics offers its vast expertise in CLIP to many clients in the pharmaceutical, medical device and biotech industries. We are eager to learn of our customers' specific CLIP requirement and to facilitate their research and product development.

http://www.creative-diagnostics.com/Cross-Linking-and-Immunoprecipitation-CLIP.html