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The Sequenom MassARRAY® System offers MALDI-TOF mass spectrometry for high accuracy and sensitivity, robust chemistry for reproducible results, and advanced data analysis software to meet the needs of any genomic laboratory. This system utilizes a homogenous MassExtend (hME – single base extension) reaction termed iPLEX GOLD, for analysis of SNPs in multiplex format. Following the MassExtend reaction, oligonucleotides are printed onto specialized Spectro CHIPs and analyzed by the Sequenom mass spectrometer. The mass spectrometer provides a high level of sensitivity, capable of detecting allele frequencies as low as 3% on average.

Sequenom Custom SNP Genotyping Service Procedures

Service Range

• Design of customized SNP panels using your content

• Oligo manufacture and pool balancing

• Running of iPLEX chemistry

• Spot and fire of SpectroCHIP using MassARRAY System

• Analysis of genotypes and delivery of genotype report

Advantages

• Very fast assay design: a new assay can be designed within a few hours. In addition, a semi-automated workflow and unmodified oligos provide rapid turnaround times

• Highly precise and quantitative detection

• High degree of multiplexing

• High SNP conversion rates: >95%

• Assay works well with degraded DNA from formalin-fixed paraffin embedded (FFPE) tissue

http://www.cd-genomics.com/MassARRAY-SNP-Genotyping-Service.html