Phage Display Library Construction

Scientists from Creative Biolabs are professional in construction of various phage display libraries, including cDNA libraries, peptide libraries and scFv/Fab antibody libraries. We normally generate libraries with a diversity of n×10⁸; we are able to generate libraries of n×10^9-10. In case you need a special cDNA, peptide or antibody library, you can rely on our extensive experience. For peptide libraries with inserts longer than 8-mer [and up to 30-mer], we usually use one of three nucleotide doping strategies [NNK, Split-mix-split synthesis or Tri-mer codon] to maximize the amino acid sequences encoded by the inserted DNA sequences. We can make the both pIII-fusion libraries and pVIII-fusion libraries. For scFv/Fab library construction, we can use both human plasma cells and splenocytes from naïve or immunized mice. We are also professional in producing fully or semi-synthetic antibody libraries.

Phage display systems can be grouped into two classes based on the vector system.

    Library types:
• Phage display peptide libraries;
• Phage display antibody libraries, including immunized antibody libraries, naïve antibody libraries and semi-synthetic/synthetic antibody libraries.
• cDNA phage display libraries

http://www.creative-biolabs.com/phage-display-library-construction.html