Phage Display Library Screening

Our staff scientists have extensive experience in screening of phage display peptide, cDNA and scFv/Fab libraries. In particular, by conducting library screening, panning, for 4 cycles, we normally get scFv/Fab antibodies of an affinity of 10^-7 By constructing serial sub-libraries of the isolated scFv/Fab antibodies, Creative Biolabs' proprietary protocol allows increase of the affinity of the scFV antibodies from 10^-8 to 10^-9. We have successfully obtained a scFV antibody that has an extremely high affinity of 10^-12, whose binding to the antigen is essentially irreversible.

    Phage Display Libraries:
• Libraries commercially available
• In house premade libraries (HuScL®, HuSdL®, HuFabL®, MuScL®)
• Custom constructed library or libraries from the customer

   Targets: 
• Purified antigens;
• Un-purified antigens;
• Intact cells, tissues or any in vivo selection systems required by the customer.

    Applications:
• Identify enzyme inhibitors
• Map epitopes on antigens;
• Select new antibodies;
• Discover/validate new therapeutic targets;
• Discover interaction between ligand and receptor;
• Select any peptide/protein binders

http://www.creative-biolabs.com/phage-display-library-screening.html